玉米新不育胞质WBMs小孢子败育的细胞学观察

Cytological Observation on Microspore Genesis of WBMs a New Line of S-CMS Maize

利用普通光学显微镜和透射电子显微镜研究了新发现的玉米细胞质雄性不育材料WBMs的小孢子败育过程。观察到Mo17cms-WBMs的小孢子在单核花粉中期以前发育正常,部分小孢子从单核花粉晚期质核出现异常,多数小孢子可正常发育到二核花粉期,随后开始崩溃,形成空瘪皱缩的花粉粒。雄性不育材料WBMs胞质的小孢子败育过程与S组唐徐胞质不育材料类似,败育发生晚,绒毡层等体细胞活动正常,属典型的配子体不育类型。

WBMs is a cytoplasmic male sterile line developed by Huazhong Agricultural University. The microspore genesis of WBMs was observed by optical and electron microscope. The results indicated that the microspores of WBMs could normally develop before the middle stage of microspore, but some of microspores began degradation in the late stage, and others could normally develop until the bi-nuclear pollen stage, sequentially, the pollen grain collapse suddenly, as a result, shrunken pollens were formed. The process of microspore abortion of WBMs was similar to Tangxu CMS of S group which pollen abortion is happened in late developing stage and the normally somatic activity such as tapetal cell. The sterility of WBMs was typical gametophyte sterility. According to its cytological characters, cytoplasm of WBMs can be preliminarily classified into S group of maize CMS.