兔耳增生性瘢痕模型的建立

Establishment of rabbits hypertrophic scars model

目的建立一种稳定、易复制、又能反映增生性瘢痕变化规律的动物模型。方法通过损伤日本大耳白兔耳内侧皮肤,与损伤后7天去痂;建立起增生性瘢痕的动物模型;进行9个月的大体形态和组织病理观察。结果兔耳损伤创面在经过早期的炎症反应和肉芽组织增生后,逐渐上皮化;在术后30天时,形成了色红,质硬,突出皮面的瘢痕;并在术后6个月内持续增生,9个月时仍维持其硬度厚度和颜色。90天的瘢痕组织学显示:组织水肿,淋巴细胞浸润,微血管增生,成纤维细胞大量增生,排列紊乱;胶原纤维增生,排列紊乱,局部有涡状及结状排列;网状纤维增多;弹力纤维缺失。术后6个月和9个月时,胶原束渐粗大,成纤维细胞数减少,炎症反应消失。结论采用本实验的模型复制方法,可以得到稳定,复制成功率高且能基本反应增生性瘢痕增生规律的动物模型。

Objective: To establish animal models for hypertrophic scars though creating wounds over the ventral surface of the rabbits′ ears. Methods: First, wounds were created over the ventral surface of the rabbits′ ears. on 7th day postwounding, the escha was removed. The histological changes were observed for 9 months. Results: After the inflammation, the formation of the granulation tissue, the ulcerated area epithelized step by step. On 30th day postwounding Visibly raised and palpable scars appeared, Each of the scars remained raising in 90 days or beyond, and remained the shapes on 270th day postwounding. Histologically, the scars on 90th day were noted to be thickened compared with surrounding tissue. The scars were demonstrated largely horizontally arranged collagen fibers, increased vascular tissue and mild chronic inflammation.The elevated area of the scars had irregular arranged collagen fibers, often with a circular or whirled appearance. There were also increased vascularity and proliferated fibroblasts on 180th and 270th day, the scars remained irregularly arranged collagen fibers and fibroblasts. Conclusion: Using the method of this experiment, animal models for HS could be easily replicated.