淋巴细胞亚群的功能及其辐射效应

The Function of Lymphocytes Subpopulations and Their Radiation Effects

应用淋巴细胞单克隆抗体(McAb)的Panning法或亲和层析分纯 CD_4^+、CD_8^+、B细胞和非CD_4^+细胞,也应用PHA、ConA、PWM和LPS分别激活淋巴细胞,然后用~3H-TdR按入、集落形成及羟基磷灰石方法,探讨了各亚群淋巴细胞的调节功能及其辐射效应.实验结果表明PWM能激活CD_4^+、非CD_4^+和B细胞,PWM细胞能诱导LPS细胞转化,其中一种细胞受照即失去协同作用,PWM细胞受照影响更严重.ConA能激活CD_4^+和CD_8^+细胞,两者均可抑制LPS细胞转化,并能互相增强抑制效应,受照后消失.辐射敏感性比较:ConA细胞和PHA细胞大于PWM细胞,PWM细胞大于LPS细胞,CD_8^+细胞和B细胞大于CD_4^+细胞。

The lymphocyte subsets CD_4^+, CD_8^+, B and non-CD_4^+ cells were separated by using monoclonal antibodiesand activated by PHA, Con A, PWM and LPS respectively. The methods of ~3H-TdR incorporation, colony for-mation and hydroxylapatite chromatography were used to investigate the function of lymphocyte subsets and their radiation effects. The results showed that PWM cells could activate CD_4^+, non-CD_4^+ and B lymphocytes. PWM cells can induce the transformation of LPS lymphocytes, when one kind of cells was exposed to light the synergistic function disappeared immediately, PWM cells were much sensitive to irradiation than LPS cells. Con A cells can activate CD_4^+ cells and CD_8^+ cells, both of them could suppress the transformation of LPS cells and enhanced the inhibitory effect each other, but the inhibitory effect would disappear after exposure. This study showed that the radiosensitivity of these cells was as follows: Con A and PHA cells>MPWM cells>LPS cells; CD_8^+ and B cells>CD_4^+ cells.