摘要
以D4020大孔吸附树脂为吸附剂,对油茶皂苷进行分离纯化。考察洗脱剂浓度、洗脱流速及碱液浓度对其纯化效果影响,结果研究表明:用0.2%碱液,20%、60%乙醇梯度洗脱后,油茶皂苷纯化倍数达到2.04,收率为为75%;经紫外扫描、HPLC分析比较,纯化后皂苷达到较好分离效果。
The D4020 macroporous adsorbent resins was selected to separate and purify total camellia
saponin from seeds of camellia oleifera. The effect of concentration of eluting reagent and eluting
rate were studied in the paper. The content of camellia saponin unpurified was 2.05 times lower than
the one purified by D4020 macroporous adsorbent at the following technological condition:0.2%
NaOH, 20% and 60% ethanol as eluting reagent, eluting velocity 1BV/h. After UV scanning and
HPLC analyzying, the results also showed that D4020 macroporous adsobent resin could well sepa-
rate camellia saponin under the conditions listed above, with a total camellia saponin recovery rate of
over 75%.
出处
《粮食与油脂》
2004年第11期19-21,共3页
Cereals & Oils
关键词
D4020大孔吸附树脂
油茶皂苷
分离
D4020 macroporous adsorbent resins
camellia saponin
separation