摘要
本文探索了利用碱浸提和酶法去蛋白从赤魟软骨中提取分离纯化粘多糖的技术,对多糖含量进行了测定。实验研究表明:当NaOH浓度为6%,40℃提取6h,利用中性蛋白酶在40℃下酶解7h,三氯乙酸沉淀去蛋白,加入4倍体积的95%乙醇沉淀多糖时,粘多糖提取率可达21.84%,产品纯度约83.52%,为白色粉末。
The technique of refining glue polysaccharide from Skate’s cartilage is studied and the content of gluepolysaccharide is measured.The experiment study shows: When the content of NaOH is 6%, temperature is 40℃, extractingtime is 6h,the time of neutral protein enzyme dissolution is 7h under 40℃, Trichloroacetic acid precipitate & separate protein,4 times volume 95% C2H5OH precipitate glue polysaccharide,the extracting rate is over 21.84%, the product is white powderand purity is about 83.52%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第11期135-137,共3页
Food Science
