黑甜玉米中黑色素提取及纯化工艺研究

Study on Black Pigment Extraction and Purification Technology of Black Sweet Corn

以黑甜玉米为原料,通过正交试验研究了诸多因素对提取色素效果的影响。试验结果表明,影响色素提取效果的主要因素是酸度,以70%的乙醇与0.1mol/L HCl (pH=2)混合提取剂,固液比为1:5,在70℃下浸提120min,共二次效果最佳。通过硅胶层析纯化后,色素纯度明显提高。

The black sweet corn was used as raw material in this experiment to extract pigment,the effect of different factorson extraction of pigment with orthogonal design test was investigated.The results showed that among the tested factors acidityis the main influenced factors,and the optimum condition was that the extractive reagent was mixture in which 70% alcohol and0.1mol/L HCl (pH=2), the weight of mixture was 5 times as large as that of the black corn,the black sweet was extracted two timesat 70℃,each step for 120min.Purified by silica gel column chromatography, the purity of product is higher.