多重PCR方法对大豆转基因食品的定性检测

Qualitative Detection of Genetically Modified Soybean in Food by Multiplex PCR Analysis

为了同时检测转基因食品中所含的多个目标基因序列,本文采用多重PCR分析技术对转基因大豆食品进行了检测。为了排除扩增结果的假阴性,大豆外源凝集素基因和肌动蛋白基因被作为内部对照以评价所有PCR反应的效率。当转基因大豆含量仅为0.15%时,仍然可以对转基因食品进行可靠的鉴定,从而表明该方法的高度敏感性。该文所述的多重PCR系统是一种简单、准确并且敏感的检测方法,只需要进行一个反应就可以检测多个目标基因序列,因而可以用来对转基因原材料及加工成品进行高精度、高灵敏的检测。

Multiplex PCR procedures were developed for simultaneously detection multiple target sequences in geneticallymodified soybean. In order to eliminate any false negatives results, lectin and β-actin genes in soybean were included asinternal control targets to assess the efficiency of all reactions. Reliable identification of genetically modified soybean was stillachieved when its content was 0.15%, indicating high levels of sensitivity. The systems described herein is one of simple,accurate and sensitive detection methods in which only one reaction was necessary to detect multiple genetically modified targetsequences that could be reliably used for routine analysis of raw and processed food production.