藤茶多糖的抗氧化作用研究

The Anti-oxidative Effect of Polysaccharide from Ampelopsis Grossedentata

目的: 探讨藤茶多糖的体内外抗氧化作用。 方法: (1)测定藤茶多糖(Ampelopsis Grossedentata polysaccharide,AGP)的还原能力;(2)测定AGP在化学模拟体系、体外血清的抗活性氧能力;(3)测定AGP对小鼠肝匀浆、小鼠肝线粒体MDA生成的影响;(4)用分光光度法测定H2O2诱导小鼠红细胞溶血和Fe2+-VC诱导的肝线粒体肿胀程度来研究AGP的抗氧化效果;(5)小鼠随机分成对照与AGP组,腹腔注射AGP 150mg/kg·bw·d 12d后,测定小鼠血清抗活性氧能力及肝组织MDA含量。 结果: AGP具有一定的还原能力,一定浓度范围内具有显著的体外抗活性氧能力并能抑制小鼠肝组织及肝线粒体MDA的生成,可减少红细胞诱导溶血和肝线粒体肿胀程度,对小鼠体内肝组织MDA生成的抑制作用显著。 结论: AGP一定浓度范围内具有抗氧化作用。

Objective: To probe into the antioxidation mechanism of Ampelopsis Grossedentata polysaccharide(AGP)in vitro andvivo. Methods:(1)The deoxidazation of AGP was measured to analyse the antioxidation activity of it;(2)The scavenging effect ofAGP on active oxygen in modified chemical systems and in mice serum were detected ;(3)The content of MDA in mice liver andliver mitochondria in vitro were observed; (4)The hemolysis of mice RBC and the swelling of mitochondria induced by Fe2+-VC weredetermined by spectrophotometric methods; (5) The mice were randomly divided into control group and AGP group. After ip150mg/kg·d AGP for 12d,the effect of AGP on active oxygen in mice serum and MDA in mice liver were measured. Results:In vitro, AGP had deoxidazation effect, could scavenge active oxygen , inhibit the formation of MDA in mice liver and mitochondria,the hemolysis of mice RBC and the swelling of mice liver mitochondria . In vivo, there was a significant difference between thecontrol group and the AGP group(ip 150mg/kg·d)in MDA value of mice liver homogenate.Conclusion: AGP has antioxidationeffects within a range of certain concentration.