猪口蹄疫O型病毒ORMF8测毒试验

Virulence detection of porcine foot and mouth disease virus of ORMF8 in type O

用中国农科院兰州兽医研究所统一提供的猪口蹄疫O型灭活疫苗效检攻毒用种毒ORMF8,在3个企业同时用架子猪进行测毒,分别测得猪的最小感染量(MID)和猪的半数感染量(ID50)。3家单位测得的MID和ID50分别为10-6.0/2mL,10-8.2/2mL;10-7.0/2mL,10-7.0/2mL;10-6.0/2mL,10-7.5/2mL。将所有实验动物统计计算,MID约为10-6.3/2mL,ID50约为10-7.6/2mL。从这些数据得出,该试验1MID约为20ID50。

The virulence of foot and mouth disease(FMD) virus of ORMF8 in type O used as the challenge virus when potency tests of porcine FMD vaccine was detected on pigs. The minimal infecting dose(MID) and the (median) infective dose(ID_(50)) were detected by three factories. The MID and ID_(50) determined by Lanzhou Veterinary (Research) Institute, Stock Lanzhou Biological Products Factory and Inner Mongolia Biological Products Factory of Jinyu Group were 10^(-6.0)/2 mL, 10^(-8.2)/2 mL; 10^(-7.0)/2 mL, 10^(-7.0)/2 mL; 10^(-6.0)/2 mL, 10^(-7.5)/2 mL respectively. The statistical results showed MID was 10^(-6.3)/2 mL and ID_(50) was 10^(-7.6)/2 mL. As a result, 1 MID was (equal) to 20 ID_(50) approximately in this test.