流感病毒灭活疫苗新型佐剂——壳聚糖增强免疫作用研究

Enhancement of Antibody Response by Chitosan,A Novel Adjuvant of Inactivated Influenza Vaccine

目的 检测壳聚糖作为流感病毒灭活疫苗佐剂的效果。方法 将壳聚糖与疫苗混合经腹腔免疫BALB c小鼠 ,间隔 3周 ,免疫 2次。免疫后取血清 ,检测血清中所产生的IgG、IgG1、IgG2a等抗体水平 ,同时 ,取小鼠鼻洗液检测IgA抗体 ;加强免疫后 1周 ,用致死性 (40LD50 )流感病毒A PR 8 34(H1N1)攻击小鼠 ,观察小鼠的体重变化和保护作用。结果 壳聚糖作佐剂能显著增强血清抗体含量 ,并提高小鼠抗病毒攻击的能力。结论 壳聚糖作为流感病毒灭活疫苗的新型佐剂 ,可以增强疫苗的抗体反应。

Objective To study the effect of chitosan as an adjuvant of inactivated influenza vaccine. Methods Inoculate i.p. BALB/c mice with 2 doses of inactivated influenza vaccine containing chitosan as an adjuvant,at an interval of 3 weeks.D etect the sera of mice for IgG,IgG1 and IgG2a levels,and nasal washing for IgA l evel.Challenge the mice with a lethal dosage (40LD 50) of influenza virus A /P R/8/34(H1N1) one week after the second dose of vaccine (booster) was given,obser ve the change of body weights and evaluate the protective effect of vaccine. Results The serum antibodies of mice increased significant ly,and the protection against challenge with influenza virus was enhanced. Conclusion As a novel adjuvant of inactivated influenza vaccine ,chitosan may enhance the level of antibody response.