双酚A雄性生殖毒性的体内外实验研究

Experimental studies on male reproductive toxicity of bisphenol A in vitro and vivo

目的探讨双酚A对雄性动物生殖机能的影响。方法将双酚A混入饲料(0、1和5g/kg)连续饲喂成年32只SD大鼠14d,放免法测定睾酮和雌二醇并进行右睾组织形态分析;对原代培养的支持细胞染毒(0、10-7、10-6、10-5、10-4mol/L)。结果5g/kg的双酚A组的右睾平均重量1.53g显著低于对照组162g,但1g/kg组与对照组差异无统计学意义。形态观察发现,2个双酚A组中的曲细精管基底膜均与生精细胞分离;部分生精细胞和支持细胞发生核固缩和空泡变性;同时,双酚A处理使黏附于支持细胞的生精细胞平均数量由对照组的794个分别减少为413和304个。此外,双酚A在体内外实验中均抑制睾丸支持细胞的波形蛋白表达,阻碍细胞骨架和胞间联结的形成,使支持细胞在体外培养中的形态变得异常细长。但双酚A对血清雌二醇和睾酮浓度的影响没有统计学意义。结论双酚A可能通过破坏支持细胞骨架和改变支持细胞形态而损害雄性生殖功能。

Objective To explore the effects of Bisphenol A in adult rats and its possible mechanisms Methods BPA (in corn oil) was administered orally to 9 week old male Sprague Dawley rats for 14 days (0, 1 and 5g/kg bw), and incubated primary Sertoli cells from pubertal SD rats with 0, 10 -7 , 10 -6 , 10 -5 , 10 -4 mol/L BPA Results After oral administration, a significant decrease in right testis weight was observed in 5g/kg dose group, but not in the 1g/kg bw dose group Germ cells were detached from basement membrance of seminiferous tubules and Sertoli cells in BPA treated groups Administration of BPA at 1 g/kg bw and 5 g/kg bw produced both nucleus pycnosis and vacuolized nucleus in germ cells and Sertoli cells A marked loss in vimentin staining in Sertoli cells from testis of BPA treated rats was detected No change in levels of serum estradiol and testosterone was observed after two week exposure to BPA In Sertoli cell primary culture, BPA destroyed the cytoskeleton and cell cell junctions, and elongated Sertoli cells Conclusion These results suggest that BPA may injure reproductive function of male rats by destroying the cytoskeleton and changing the form of Sertoli cells