急性敌百虫中毒与氧化应激及自由基损伤的关系

Relationship between acute dipterex poisoning and oxidative stress and free radical damage

目的探讨急性敌百虫中毒与氧化应激和自由基损伤的关系。方法采用随机对照设计,用分光光度分析法检测了2002年7月至2003年6月抢救的82例急性敌百虫中毒者和92例健康成人志愿者的血浆维生素C、维生素E、红细胞过氧化脂质(LPO)水平和超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)活性。结果中毒组的维生素C为(3735±998)μmol/L,维生素E为(1657±454)μmol/L,SOD为(1785±154)U/gHb,AChE为(2131±576)U/gHb,志愿组的维生素C为(5534±1598)μmol/L,维生素E为(2566±724)μmol/L,SOD为(2124±185)U/gHb,AChE为(3053±836)U/gHb,中毒组上述指标均显著低于志愿组;中毒组的LPO为(3520±529)nmol/gHb,显著高于志愿组的(2787±466)nmol/gHb。控制年龄的偏相关分析结果表明,中毒组的AChE值与LPO值呈负相关,与维生素C、维生素E和SOD值呈正相关。结论急性敌百虫中毒者体内存在着严重的氧化应激和自由基损伤。

Objective To study relationship between acute dipterex poisoning and oxidative stress and free radical damage Methods Eighty two patients with acute dipterex poisoning (ADPP) and ninety two healthy adult volunteers (HAV) were enrolled in the study with randomized controlled trial design Plasma levels of vitamin C (VC) and vitamin E (VE), as well as level of lipoperoxide (LPO) and activities of superoxide dismutase (SOD) and acetylcholinesterase (AChE) in the red blood cells (RBC), were determined by spectrophotometry Results Levels of VC and VE, and activities of SOD and AChE were (37 35±9 98) μmol/L, (16 57±4 54) μmol/L, (1 785±154) U/g Hb and (213 1±57 6) U/g Hb, respectively, in the ADPP group, significantly lower than those in the HAV group, (55 34±15 98) μmol/L, (25 66±7 24) μmol/L, (2 124±185) U/g Hb and (305 3±83 6) U/g Hb, respectively Plasma level of LPO was (35 20±5 29) nmol/g Hb in the ADPP group, significantly higher than that in the HAV group, (27 87±4 66) nmol/g Hb Partial correlation analysis suggested that there existed negative correlation between activity of AChE in the RBC and plasma level of LPO ( r =-0 274, P =0 013) and positive correlation between activity of AChE in the RBC and plasma levels of VC and VE, and activity of SOD in the RBC ( r =0 333, P =0 002, r =0 269, P =0 015 and r =0 248, P =0 026, respectively) in the ADPP, adjusted for age Coefficient of reliability alpha was 0 682 ( P <0 001), with a standardized alpha of 0 868 ( P <0 001) Conclusion There exist severe oxidative stress and free radical damage in patients with acute dipteres poisoning