ST-1抗豚鼠和兔心肌缺血再灌注损伤的血流动力学研究

Effects of ST-1 on myocardial dysfunction and hemodynamics induced by ischemia reperfusion

目的 :拟证实双萜类化合物ST 1的抗心肌缺血再灌注损伤作用。方法 :(1)Langendorff装置逆向豚鼠心脏灌注 ,预先灌注ST 11.0、5 .0和 10 μmol·L- 1 5min ,停灌 3 0min ,再灌注 2 0min ,制成豚鼠心肌缺血再灌注损伤模型 ,观察药物对心脏舒缩功能、冠脉流出液量的影响 ;(2 )结扎麻醉兔冠脉左前降支 3 0min后再连续灌注 60min ,制成兔心肌缺血再灌注损伤模型 ,ST 12 5～ 10 0 μg·kg- 1 静脉注射 ,动态观察其对血流动力学指标的影响。结果 :ST 1预处理可有效减轻离体豚鼠缺血再灌注引起的左室舒缩功能下降 ;兔ST 1组左心室等容期压力最大变化速率、左心室收缩压、左心室发展压、左心室压峰值时间的变化值明显小于模型对照组 (P <0 .0 5 ) ,心率、舒张压、收缩压和平均动脉压变化不明显 (P >0 .0 5 )。结论 :ST 1预处理有抗离体豚鼠和在体家兔心肌缺血再灌注损伤的作用。

Objective To study the effects of ST-1,a diterpen e c ompound, on myocardial dysfunction induced by ischemia reperfusion.Meth ods The isolated guinea-pig heart was perfused with modified Tyrde bu ffer solution by Langendorff. ST-1 1,5,10-μmol·L -1 were perfused 10 min prior to ischemia, ischemia 30 min and reperfusion 20 min in vitro,respecti vely. The changes of hemodynamics were observed in rabbits with m yocardial ischemia reperfusion model induced by occluding the left anterior desc ending coronary artery(LAD) for 30 min followed by 60 min reperfusion.R esults The preconditioning with ST-1 could effectively preserve the ca rdiac function.The values of change in LVSP,LVDP',±dp/dt max,T-dp/dt m a x induced by ST-1 were significantly less than the respective value of vehi c le control inanesthetized and ischemia reperfused rabbits.There were no signifi can t changes of heart rate and blood pressure by administration of ST-1.Co n clusion Preconditioning with ST-1 could ameliorate cardiac dysfunction induced by ischemia and reperfusion significantly.