TGF-β1及p27在原发性胆囊癌中的表达及临床意义

Expression and Clinical Significance of TGF-β1 and p27 in Gallbladder Carcinoma

目的 检测TGF β1及 p2 7在原发性胆囊癌中的表达 ,研究其蛋白产物在原发性胆囊癌发生、发展过程中的表达特点及相互关系。方法 应用免疫组化SP法检测 36例原发性胆囊癌中TGF β1及p2 7的表达 ,并以同期2 0例慢性胆囊炎作对照。结果 36例原发性胆囊癌组织中 2 3例TGF β1基因蛋白产物呈阳性表达 ,表达阳性率为6 3.9% ,高于对照组的 10 .0 % (P<0 .0 5 ) ;17例p2 7蛋白阳性表达 ,表达阳性率为 4 7.2 % ,显著低于对照组的 10 0 %(P<0 .0 5 )。TGF β1在有淋巴结或远处转移及NevinⅣ～Ⅴ期患者中表达阳性率 (均为 79.1% )明显高于无转移及NevinⅠ～Ⅲ期的患者 (均为 33.3% ) ,P<0 .0 5 ;p2 7在高、中分化、无淋巴结或远处转移及NevinⅠ～Ⅲ期的患者中表达阳性率分别为 6 0 .9%、75 .0 %及 75 .0 % ,明显高于低分化、有转移及NevinⅣ～Ⅴ期患者 (2 3.0 %、33.3%及 33.3% ) ,P<0 .0 5。p2 7与TGF β1表达两者呈负相关 (r =- 0 .4 4 73,P<0 .0 5 )。TGF β1阳性患者生存率低于TGF β1阴性患者 ,差异有显著性意义 (P<0 .0 5 ) ;p2 7阳性患者生存率高于 p2 7阴性患者 ,差异有显著性意义 (P <0 .0 5 )。结论 在原发性胆囊癌细胞中TGF β1表达上调 ,p2 7表达下调 。

Objective To investigate the expression of tumor growth tactor β1 (TGF-β1) and p27 in gallbladder carcinoma and their relation to the development of the carcinoma. Methods The expression of TGF-β1 and p27 in 36 cases of gallbladder carcinoma was detected by SP immunohistochemical staining. Twenty cases of chronic cholecystitis were collected as control. Results The positive rate of TGF-β1 (63.9%) was higher than that of the control (10.0%), P <0.05, and the positive rate of p27 (47.2%) was lower than that of the control 100%( P <0.05). The positive rate of TGF-β1 was significantly higher in metastasis or Nevin Ⅳ～Ⅴ stage cases than that of non-metastasis or Nevin Ⅰ～Ⅲ stage cases 33.3% ( P <0.05). The positive rate of p27 was statistically higher in moderate and highly differentiation (60.9%), non-metastasis (75.0%) or Nevin’s Ⅰ～Ⅲ stage (75.0%) cases than those of poor differentiation (23.0%), metastasis (33.3%) and Nevin Ⅳ～Ⅴ stage (33.3%) cases ( P <0.05). The expression of p27 was negatively correlated with that of TGF-β1( r =-0.4473, P <0.05). There was significant difference in survival time between patients with TGF-β1 positive and TGF-β1 negative( P <0.05). The difference was also found between patients with p27 positive and p27 negative. Conclusion The upregulation of TGF-β1 and downregulation of p27 in gallbladder carcinoma indicates the imbalance of TGF-β1/p27 system, which may play a role in the carcinogenesis and predict the malignant behaviors of the carcinoma.