MRP-1/CD9基因在胃癌及其癌前病变中的差异表达

Differential expression of motility-related protein-1/CD9 gene in gastric cancers and premalignant lesions

目的 筛选和鉴定与胃癌发生、发展相关的基因片段 ,探讨胃癌的癌变分子机理。方法 应用荧光 m RNA差异显示技术对胃正常黏膜、癌前病变及癌组织的基因差异表达情况进行了分析。对其中的运动相关蛋白 (motility- related protein- 1,MRP- 1/CD9)基因 ,用 Northern印迹及逆转录 -聚合酶链反应技术分析在正常及不同病理状态下该基因表达的情况。结果 Northern印迹结果与 m RNA差异显示的结果相符合 ,逆转录 -聚合酶链反应显示 MRP- 1/CD9在胃癌组织中的表达 (0 .31± 0 .18)低于相应的正常胃黏膜 (0 .4 9± 0 .2 4 )及癌前病变组织 (0 .4 7± 0 .18) ,差异有显著性 (P<0 .0 5 ) ;在胃癌中 ,MRP- 1/CD9在弥漫型胃癌中的表达 (0 .2 2± 0 .17)明显低于肠型胃癌 (0 .38± 0 .16 ) (P<0 .0 5 )。结论 与正常胃黏膜及癌前病变组织相比 ,MRP- 1/CD9基因在胃癌组织中表达明显降低 ,提示 MRP- 1/CD9基因的表达情况可能与胃癌的发病及其组织学分型有关。

Objective To clone and identify gastric cancer-related genes and explore the possible pathogenic mechanism of gastric cancer. Methods The differentially expressed cDNA bands were isolated by fluorescent mRNA differential display in gastric cancer specimens, matched normal gastric mucosa and premalignant lesions. The motility-related protein-1( MRP-1/CD9)gene was one of the down-regulated genes. MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Results MRP-1/CD9 expression was down-regulated in all gastric cancer tissues. Northern blot analysis confirmed this differential expression. RT-PCR analysis showed that the MRP-1/CD9 gene expression was much lower in gastric cancers(0 31±0 18) than in the matched normal gastric tissue(0.49±0.24) and in the premalignant lesions(0.47±0 18)(P<0.05), and its expression in intestinal type gastric cancer(0.38±0.16) was higher than that in diffuse type gastric cancer(0.22±0.17)(P<0.05). Conclusion The MRP-1/CD9 gene expression was down-regulated in gastric cancer, its expression was probably related to the carcinogenesis and histology types of gastric cancer.