摘要
Objective: To observe the effects of Gu Fu Sheng Capsule (骨复生胶囊 GFSC) on nitric oxide (NO) level and fibrinolytic activity in rabbits with glucocorticoid-induced ischemic necrosis of femoral head (GINFH)so as to explain the therapeutic mechanism of GFSC Methods: 48 rabbits were randomly divided into 4 The models of GINFH were produced by injection of glucocorticoid hormone for 8 weeks. From the 9th week, GFSC decoction was given to the rabbits of the group A (45g. kg-1 d-1) and MSGP extract was given to the group B (0.17 g kg-1 d-1) and the same volume of normal saline to group C and D by intragastric infusion. The content of plasma nitric oxide (NO), and activities of tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor (PAI) were detected after the hormone was injected for 4 and 8 weeks and after the drugs were given for 4 weeks. Results: Compared with the group D, the contents of NO and t-PA activity in the group C decreased significantly (P＜0.01), but the activity of PAI increased significantly (P＜0.01). Compared with the group C, the contents of NO and t-PA activity in the group A and B increased significantly (P＜0.05), but the activity of PAI decreased significantly (P＜0.05), with no significant difference between the two treatment groups (P＞0.05). Conclusion: GFSC could increase the level of NO, protect endotheolial cells and improve fibrinolytic activity, which might be the mechanism of GFSC in curing GINFH.
Objective:To observe the effects of Gu Fu Sheng Capsule(骨复生胶囊 GFSC)on nitric oxide(NO)level and fibrinolytic activity in rabbits with glucocorticoid-induced ischemic necrosis of femoral head(GINFH) so as to explain the therapeutic mechanism of GFSC.Methods:48 rabbits were randomly divided into 4 groups:GFSC group A,Ma Shi Gu Pian(马氏骨片 MSGP)group B,model group C,and control group D. The models of GINFH were produced by injection of glucocorticoid hormone for 8 weeks.From the 9th week,GFSC decoction was given to the rabbits of the group A(45g·kg^(-1)·d^(-1))and MSGP extract was given to the group B(0.17 g kg^(-1)·d^(-1))and the same volume of normal saline to group C and D by intragastric infusion.The content of plasma nitric oxide(NO),and activities of tissue-type plasminogen activator (t-PA),and plasminogen activator inhibitor(PAI)were detected after the hormone was injected for 4 and 8 weeks and after the drugs were given for 4 weeks.Results:Compared with the group D,the contents of NO and t-PA activity in the group C decreased significantly(P<0.01),but the activity of PAI increased significantly(P<0.01).Compared with the group C,the contents of NO and t-PA activity in the group A and B increased significantly(P<0.05),but the activity of PAI decreased significantly(P<0.05),with no significant difference between the two treatment groups(P>0.05).Conclusion:GFSC could increase the level of NO,protect endotheolial cells and improve fibrinolytic activity,which might be the mechanism of GFSC in curing GINFH.
