摘要
目的 :探讨个体基因识别卡的应用价值。方法 :将PCR -STR分型技术和信息技术结合 ,为 12 0 6名独生子女制作 16位点个体基因识别卡 ,并建立相应人群DNA数据库。结果 :上述人群中 ,共检出 16 8个等位基因 ,6 6 2种基因型 ,基因型分布符合Hardy-Weinberg平衡定律 (P >0 .0 5 )。多态信息量 (PIC)为 0 .5 816～ 0 .86 0 9,累计非父排除率 (PPE)和个体识别力 (DP)分别达 0 .999996 0 6 36 2 8316和 0 .9999999999999999。结论 :制成 16位点个体基因识别卡具有强大的个体识别能力 ,预期可在失散追寻、财产继承、骨髓移植等需要进行个体识别的活动中发挥重要的作用 ;所建立的DNA数据库可为福建省汉族人群法医个体识别和亲权鉴定、遗传学研究提供基础资料。
Objective: To evaluate the function of genetic identification cards. Methods: The genetic identification cards of 1206 children that came from single -child family were prepared by PCR-STR technique combined with information technique. Results: 168 alleles and 662 genotypes were checked out among the above subjects. The frequencies of alleles revealed no significant deviation from the Hardy-Weinberg equilibrium. Polymorphism information content (PIC) were 0.5816-0.8609. The accumulated discrimination power(DP)and the accumulated probability of paternity exclusion(PPE) were 0.9999999999999999 and 0.999996063628316, respectively. Conclusion: The genetic identification cards of 16 loci have stronger individual identification and can play an important role in some activities such as researching lost properties, property inheriting and marrow transplanting. DNA data are useful markers for individual identification and paternity test in the fields of forensic science and genetics.
出处
《中国计划生育学杂志》
2004年第11期673-676,共4页
Chinese Journal of Family Planning
基金
福建省科技厅社会发展基金项目资助 (2 0 0 3Y0 37)
