摘要
目的 :采用反相高效液相色谱法对感冒一小时胶囊中羌活、独活的指标成分异欧前胡素、蛇床子素同时测定。方法 :采用HypersilC18柱 ,流动相为乙腈 水 (4 2 5∶5 7 5 ) ,检测波长 32 0nm。结果 :线性范围异欧前胡素 0~0 3975 μg,蛇床子素 0~ 0 382 5 μg ,相关系数异欧前胡素 0 9999,蛇床子素 0 9997,平均回收率异欧前胡素98 1% ,蛇床子素 99 8%。结论 :方法简便、准确、灵敏可靠 ,适用于制剂及药材中异欧前胡素和蛇床子素的分析测定。
Objective: To develop a method for the determi na tion of isoimperatorin and osthol in Ganmao Yixiaoshi capsule by RP-HPLC. Method: The chromatographic conditions included Hypersil C 18 c olumn and the mobile phase consisting of a mixture of acetonitrile-water (42.5 ∶57.5). The detection wavelength was at 320 nm. Result: The calibration curve was linear over the concentration range of 0~0.397 5 μg for isoimperatorin and 0~0.382 5 μg for osthol respectively. The correla tion coefficient was r=0.999 9 and r=0.999 7. The average recovery of isoimperatorin was 98.1 % and that of osthol was 99.8%. Conclusion: The method sensitive, simple and accurate for the determination of iso imperatorin and osthol concentrations in Ganmao Yixiaoshi capsule. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第10期950-952,共3页
China Journal of Chinese Materia Medica