HPLC测定南葶苈子中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的含量

Determination of quercetin-3-O-β-D-glucopyranosyl -7-O-β-D-gentiobioside in Semen Descurainiae by HPLC

目的 :研究南葶苈子中槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷的含量测定方法 ,建立南葶苈子药材质量标准。方法 :采用反相高效液相色谱法 ,以自制标准品作对照 ,对南葶苈子药材中主要有效成分槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷进行了含量测定。 结果 :回收率为 99.78% ,RSD为 2 .4 %。结论 :该方法可用于南葶苈子药材的质量控制。

Objective: To establish a method for the d et ermination of quercetin-3-O-β-D-glucopyranosyl-7-O-β-D -gentiobioside in Semen Descurainiae. Method: HPLC was used with self-made quercetin-3-O-β-D-glucopyranosyl-7-O- β-D-gentiobioside as reference substances. Result: The average collection was 99.78%, RSD 2.4%. Conclution: T he method is appropriate for quality control of Semen Descurainiae. [