肿瘤血管内皮细胞和成纤维细胞表达血管形成因子

Study on the expression of angiogenic factors by tumor fibroblasts and endothelial cells

目的 :探讨肿瘤血管内皮细胞和成纤维细胞在肿瘤血管形成中的作用。方法 :对肿瘤血管内皮细胞和成纤维细胞模型ECV304 -SKOV3和L929-H22 细胞 ,用免疫细胞化学测定促进血管形成因子的表达、RT -PCR测定其端粒酶活性 ,并与亲代细胞比较。结果 :ECV304 -SKOV3细胞和L929-H22 细胞的端粒酶活性明显强于亲代细胞ECV304、L929,分别为0.778±0.011、0.875±0.026、0.692±0.014、0.684±0.012(P<0.001)。ECV304 -SKOV3细胞表达血管形成因子MMP -9、bcl -2增强 (P<0.05) ,TN减弱(P<0.05) ;L929-H22 细胞表达MMP -9、TGF -β1、TN、bcl-2增强 (P<0.01) ;二者表达PCNA增强 (P<0.01)。结论 :肿瘤血管内皮细胞和成纤维细胞存活能力、血管形成因子表达增强 ,可能参与肿瘤血管生成。

Objective:To explore the role of vascular endothelial cells and fibroblasts derived from tumors in the tumor angiogenesis in solid tumors.Methods:Immunocytochemistry and RT-PCR were respectively used to detect the expression of MMP-9,TGF-β1,TN, bcl-2,PCNA,and tolomerase activity of ECV304-SKOV3 cells and L929-H22 cells,which were established as the cell models for vascular endothelial cells and fibroblasts derived from tumors in our laboratory before. Results:Telomerase activity level of ECV304-SKOV3 cells was higher than that of ECV304 cells (0.778±0.011 vs 0.692±0.014,P<0.001),so was L929-H22 cells and L929 cells (0.875±0.026 vs 0.684±0.012,P<0.001).ECV304-SKOV3 cells expressed MMP-9,bcl-2,and PCNA stronger(P<0.05),while L929-H22 cells expressed them all stronger(P<0. 01).Conclusion:The results suggest that vascular endothelial cells and fibroblasts derived from tumors survive longer and express angiogenic factors stronger,which may promote Angiogenesis in tumors.