革兰阴性杆菌中VEB-1型超广谱β-内酰胺酶的分布情况调查

Epidemiological survey of VEB-1 Extended-Spectrum β-Lactamases in Gram negative bacteria

目的 分析上海第二医科大学附属瑞金医院 (以下简称瑞金医院 )和上海交通大学附属第六人民医院(简称市六医院 )分离的 340株革兰阴性杆菌中VEB 1型超广谱 β 内酰胺酶 (ESBL)的分布 ,并分析瑞金医院灼伤科患者标本中分离的产VEB 1型ESBL铜绿假单胞菌是否有同源性。方法 用聚合酶链反应 (PCR)扩增细菌的veb 1基因 ,用PCR RFLP检测并分型整合子 ,以ERIC2为引物 ,采用随机引物扩增多态性DNA分型法(RAPD)对瑞金医院灼伤科分离到的 16株产VEB 1型ESBL的铜绿假单胞菌进行同源性分析。结果 市六医院分离的菌株中未检测到veb 1基因 ,瑞金医院仅在灼伤科来源的铜绿假单胞菌中检出VEB 1型ESBL ,且这些菌株用RAPD分析具有同源性。结论 瑞金医院灼伤科流行携带veb 1型ESBL基因的铜绿假单胞菌。

Objective To analyze the distribution of VEB-1 Extended-Spectrum β-Lactamases(ESBL) in 340 isolates of Gram negative bacteria isolated from Ruijin hospital and Shanghai 6th people's hospital, and verify if the 16 isolates of Pseudomonas aeruginosa with VEB-1 gene isolated from burn ward of Ruijin hospital have the same origin. Methods PCR was performed to detect the VEB-1 gene, PCP-RFLP was used to detect and classify the integron, Romdom Amplitication of Dolymorphic(RAPD) with the primer ERIC2 was used to type the 16 isolates of Pseudomonas aeruginosa with VEB-1 gene isolated from burn ward of Ruijin hospital. Results VEB-1 ESBL was not detected in bacteria isolated from Shanghai 6th people's hospital. In Ruijin hospital, VEB-1 ESBL was detected only in Pseudomonas aeruginosa isolated from burn ward and these strains had the same origin. Conclusions Pseudomonas aeruginosa with VEB-1 gene is the epidemic strain in burn ward of Ruijin hospital.