头孢西丁纸片扩散法检测耐甲氧西林金黄色葡萄球菌

Detection of methicillin-resistant S.aureus using the cefoxitin disk diffusion method

目的 评价头孢西丁纸片扩散法检测耐甲氧西林金黄色葡萄球菌 (MRSA)在临床的应用价值。方法 用头孢西丁纸片扩散法检测临床分离的 94株金黄色葡萄球菌 ,并与苯唑西林纸片扩散法、琼脂稀释法及mecA基因检测进行比较。结果 mecA基因阳性的 77株金黄色葡萄球菌 ,头孢西丁纸片扩散法均显示耐药。结论 头孢西丁纸片扩散法与mecA基因检测高度一致 ,是筛选和确认MRSA的一种可靠的试验方法。

Objective To evaluate the clinical practicability of cefoxitin disk diffusion method in detecting methicillin-resistant S.aureus (MRSA). Methods 94 strains of clinically isolated S. aureus were tested. The oxacillin disk diffusion method, oxacillin agar dilution test as well as PCR amplification of the mecA gene were performed simultaneously. Results All the 77 mecA-positive strains were identified as methicillin-resistant by cefoxitin disk diffusion method. Conclusions Cefoxitin disk diffusion method can be used reliably in detecting and confirming MRSA.