肿瘤坏死因子α基因启动子多态性与乙型肝炎易感性的相关性分析

Study on the relationship between the genetic polymorphisms of TNF α promoter and susceptibility of viral hepatitis B

目的 探讨肿瘤坏死因子α(TNFα)基因启动子多态性与乙型肝炎易感性的关系。方法 以病例对照的研究方法 ,采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)技术检测 14 6例乙型肝炎患者和 16 5名正常对照者TNFα 2 38位点和 30 8位点基因型。结果 TNFα 2 38位点G/G和G/A基因型在病例组为 94 .5 %和 5 .5 % ,对照组为 97.0 %和 3.0 % ,2组基因型和等位基因频率分布差异无显著性 (P >0 .5 )。而TNFα 30 8位点G/G和G/A基因型在病例组为 95 .2 %和 4 .8% ,对照组为 87.9%和 12 .1% ,2组基因型和等位基因频率分布差异有显著性 (P <0 .0 5 )。结论 TNFα 30 8位点G等位基因可能是乙型肝炎易感性的遗传标记 ;TNFα 30 8位点G/G基因型个体乙型肝炎病毒感染的风险率增高。

Objective To study the possible relationship between the polymorphisms of tumor necrosis factor α (TNF α) gene promoter and the susceptibility of viral hepatitis B. Methods TNF α genotypes at positions -238 and -308 were detected by PCR-RFLP in case-control study including 146 hepatitis B patients and 165 healthy controls. Results The distributions of G/G and G/A genotypes of the TNF α at position -238 hepatitis B patients were 94.5% and 5.5% whereas 97.0% and 3.0% in the controls. There was no significant difference between the two groups (P>0.05). The distributions of G/G and G/A genotypes in the TNF α at position -308 were 95.2% and 4.8% in hepatitis B patients, whereas 87.9% and 12.1% in the controls. The frequencies of alleles and genotypes of TNF α at position -308 in hepatitis B patients were significantly different from those of the controls (P<0.05). Conclusions TNF α G allele at position -308 may be one of the susceptible genetic markers of HBV infection in Han's population of China. The risk of HBV infection may increase in individuals with TNF α-308 G/G genotype.