用双抗体夹心酶联免疫吸附试验测定消化系统肿瘤患者血浆MMP-9/TIMP-2浓度的意义

Significance of the analysis of plasma matrix metalloproteimase-9/tissue inhibitor of metall oproteinase-2 concentration by sandwich ELISA in patients with digestive tract carcinoma

目的 探讨血浆基质金属蛋白酶 9(MMP 9)及基质金属蛋白酶组织抑制剂 2 (TIMP 2 )水平与消化系恶性肿瘤侵袭转移的关系。方法 采用酶联免疫吸附试验 (ELISA)测定血浆MMP 9及TIMP 2浓度。结果 81例消化系统恶性肿瘤组的MMP 9水平显著高于对照组 (P <0 .0 1) ;而血浆TIMP 2水平的升高与对照组差异无显著性。根据TNM分期 ,各组TNMⅢ～Ⅳ期者的血浆MMP 9均显著高于TNMⅠ～Ⅱ期者 ,胰腺癌TNMⅢ～Ⅳ期者的血浆TIMP 2显著升高。结论 本研究确证了文献报道的消化道肿瘤患者血浆MMP 9水平升高可能与肿瘤的侵袭转移有关。

Objective To investigate the relationship between plasma levels of matrix metallorproteinase-9 (MMP-9) or tissue inhibitor of metalloproteinase-2 (TIMP-2) and the metastasis of digestive system carcinoma. Methods Plasma MMP-9/TIMP-2 concentration was measured by sandwich ELISA in digestive tract carcinoma. Results Plasma MMP-9 levels in 81 patients with digestive system carcinoma were significantly higher than those from control group (P<0.01). The TIMP-2 level between two groups showed no difference. In tumor group, plasma MMP-9 level of TNM Ⅲ-Ⅳ stage in all kind of cancer was significantly higher than those from cancer within the TNM Ⅰ-Ⅱ stage. Conclusions A very high level of MMP-9 in plasma might be correlated to the metastasis of carcinoma.