小麦Waxy蛋白亚基缺失类型鉴定方法的研究

Studying on the Judging Technique of the Wheat Waxy Protein Sub-unites Deleting

建立适于我省面条专用小麦育种研究上应用的Waxy蛋白亚基缺失类型的鉴定技术;以改良十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术(1-D-SDS-PAGE)为基础,分析凝胶性质、试验试剂、试验条件对电泳结果的影响,获得高效低成本、分辨效果佳、重复性好的鉴定方法。

Selecting by sifting and establishing the judging technique of Waxy protein sub-unites deletion Which is used for noodle wheat breeding in Heilongjiang province. The experiment is based on improved 1-D-SDS-PAGE method . Analysing electrophoresis results which are effected by glue properties, experimental reagents and experimental conditions, and selecting a suitable judging way which is high efficiency but low cost , high segregation and good repetition.