姜黄素对Raji细胞体外抗癌作用的实验研究

Experimental Study on Anticancer Effect of Curcumin on Raji Cells in vitro

目的研究姜黄素对Raji细胞体外抗癌作用及其机制,对比研究其对Raji细胞和人单个核细胞的细胞毒性。方法用MTT法检测姜黄素对Raji细胞及人单个核细胞增殖的影响,AnnexinⅤ/PI双标流式术、缺口末端标记法检测姜黄素对Raji细胞及人单个核细胞凋亡的影响,PI单标流式细胞术测定姜黄素对Raji细胞DNA含量分布的影响。结果(1)姜黄素对Raji细胞具有明显的增殖抑制作用。(2)姜黄素可以时间和剂量依赖性方式诱导Raji细胞凋亡。(3)姜黄素组Raji细胞周期发生变化,细胞周期被阻滞于G0/G1和G2/M期,S期比例减少。(4)姜黄素对人单个核细胞无明显抑制增殖和诱导凋亡作用。结论姜黄素能够调控Raji细胞的细胞周期并诱导其凋亡,从而抑制Raji细胞增殖;姜黄素对人单个核细胞无明显细胞毒作用,而选择性作用于肿瘤细胞。

To study the anticancer effect and mechanism of curcumin on Raji cells in vitro and compared the cytotoxicities of curcumin on Raji cells and normal human peripheral blood mononuclear cell (PBMC). MethodsThe effects of curcumin on proliferation of Raji cells and human PBMC were tested by MTT assay, its effects on apoptosis of them were determined by Annexin V/PI double labeled cytometry and TUNEL, and its effects on DNA distribution in Raji cells was studied by PI single labeled cytometry. ResultsCurcumin showed marked inhibition on proliferation of Raji cell, could induce Raji cell apoptosis in time and dose dependent manner. After curcumin treatment, the cell cycle of Raji cells was blocked in G 0/G 1 and G 2/M phase and those in the S phase decreased proportionally. But curcumin showed no significant effect on inhibiting proliferation or inducing apoptosis on human PBMC. ConclusionCurcumin could regulate the cell cycle of Raji cells and induce its apoptosis, so as to inhibit its proliferation, but with no significant cytotoxicity on human PBMC. It selectively affects the tumor cell.