乙型肝炎病毒表面抗原阳性孕妇及其新生儿外周血中乙型肝炎病毒的研究

The HBV-infection status of HBsAg-positive pregnant women and their newborns

目的了解乙型肝炎病毒表面抗原(HBsAg)阳性孕妇及其新生儿外周血乙型肝炎病毒(HBV)的感染状况。方法酶联免疫吸附试验(ELISA)法检测新生儿外周血HBsAg;巢式聚合酶链反应(nPCR)检测孕妇及其新生儿外周血HBV-DNA;选择性聚合酶链反应(sPCR)检测孕妇及其新生儿外周血单个核细胞(PBMC)中HBV-DNA,三项指标任一项阳性即判定为新生儿HBV宫内感染。结果HBsAg阳性孕妇血清HBV-DNA阳性率为43.1%(53/123),PBMCHBV-DNA阳性率为30.8%(37/123);新生儿血清HBsAg阳性率为6.5%(8/123),HBV-DNA阳性率为19.5%(24/123),PBMCHBV-DNA阳性率为26.0%(32/123)。新生儿任一项阳性者50例,合计宫内感染率40.6%(50/123)。结论检测PBMCHBV-DNA对血清中HBV-DNA检测进行补充,可以比较准确地反映HBsAg阳性孕妇新生儿宫内HBV感染的状况。

Objective To learn the HBV-infection status of HBsAg-positive pregnant women and their newborns. Methods HBsAg was determined by enzyme-linked immunosorbent assay (ELISA); HBV-DNA was determined by nested PCR; PBMC HBV-DNA was determined by selected PCR; The newborns were intrauterinely infected if any one of the three markers showed positive. Results HBV-intrauterine infection rate was 43.1% for HBV-DNA and 30.8% for PBMC HBV DNA in HBsAg-positive pregnant women. HBV-intrauterine infection rate was 40.6%. HBV-intrauterine infection rate was 6.5% for HBsAg in serum, 19.5% for HBV DNA and 26.0% for PBMC HBV-DNA in newborns. Conclusion The detection of PBMC HBV is an important complement to the detectin of HBV-DNA in peripheral blood. It could be used for the aim of reflecting the the HBV-infection of HBsAg-positive pregnant women's newborns.