摘要
目的 :研究吗啡对培养的大鼠胶质细胞瘤C6的增殖抑制作用及其机制。方法 :3H -TdR掺入法测定吗啡对细胞增殖的影响 ;RT -PCR方法观察吗啡对细胞PCNA基因表达的影响。免疫组织化学法观察吗啡对细胞PCNA蛋白质水平的影响。结果 :不同浓度吗啡 (0 0 0 1- 10 0mg·L- 1 )作用于培养C6细胞 2 4h后 ,3H -TdR掺入量剂量依赖性降低。不同浓度吗啡 (0 0 0 1- 10 0mg·L- 1 )作用于培养C6细胞 2 4h后 ,细胞PCNAmRNA的相对含量与对照组相比均明显减少 (P <0 0 1)。 10mg·L- 1 吗啡作用细胞 6 - 4 8h后 ,不同时间点吗啡组细胞的PCNAmRNA的相对含量与对照组相比均明显减少 (P <0 0 1)。10mg·L- 1 吗啡作用细胞 2 4h后 ,与相邻吗啡组比较细胞内PCNA蛋白质水平明显降低 (P <0 0 1)。结论 :吗啡对胶质细胞瘤C6的增殖具有抑制作用 ,其机理可能与吗啡使细胞中PCNA的基因表达水平降低有关。
Objective: To study the effect and mechanism of morphine on proliferation of rat C6 glioma cells. Methods: 3H-TdR incorporation was used to determine the effect of morphine on cell proliferation. The effect of morphine on PCNA gene expression in C6 was determined by RT-PCR and immunohistochemistry. Results: Morphine at dose 0 001-100 mg·L -1 decreased 3H-TdR incorporation in a dose-dependent manner. The level of PCNA gene expression in C6 glioma cells decreased after exposure to morphine 0 001-100 mg·L -1 for 24 hours and also decreased at all time points after exposure to morphine 10 mg·L -1 for 6-48 hours. The protein level of PCNA in C6 glioma cells decreased after exposure to morphine 10 mg·L -1 for 24 hours. Conclusion: Morphine has an inhibitory effect on proliferation of rat C6 glioma cells and the mechanism may be related to its inhibitory effect on gene expression of PCNA.
出处
《中国药物依赖性杂志》
CAS
CSCD
2004年第3期178-182,共5页
Chinese Journal of Drug Dependence
基金
吉林省计划委员会资助课题 (No .990 5G16 8)
