摘要
建立了小黑杨转基因受体系统 ,利用农杆菌介导法将蜘蛛神经毒肽与bt基因C末端肽组成的融合基因导入小黑杨中 ,获得了 3个小黑杨转抗虫基因植株 ,转化子为A1、A2、A3,并对其进行了GUS活性及目的基因PCR检测。初步证明A1、A2、A3为转基因阳性植株。
The acceptor system of tans-gene Populus xiaohei was established, and Populus xiaohei plants transformed with fusion gene which is composed of the C terminal of bt gene and the spider insecticidal peptide were gained through agrobacterium tumefaciens transformation. The transformed plants with insect-resistance were marked as A1, A2 and A3, and GUS activity and PCR detection were conducted. The results show that the fusion gene has been transformed into the plants.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2004年第6期30-31,共2页
Journal of Northeast Forestry University
关键词
小黑杨
遗传转化
GUS
PCR
Populus xiaohei
Genetic transformation
GUS
PCR
