MB22木聚糖酶发酵条件的研究

Study on Fermentation Conditions of Xylanase by MB22

通过因素轮换试验和正交试验,对MB22菌产木聚糖酶的培养基配方和发酵条件进行了研究。结果显示最佳培养基组成是:以玉米芯粉和麸皮为碳源,麸皮120g/l,玉米芯280g/l,(NH4)2SO44g/l,CaCO31.5g/l,Tween80,2.0g/l,MgSO4·7H2O1.5g/l;最佳发酵条件为:起始pH5.0,摇床培养温度30℃,转速160r/min,振荡培养84h。在最佳发酵条件下,MB22菌的最高产酶活力可达898.23U/ml。虽然与一些高产菌株相比该菌株的产酶能力还有待于进一步提高,但该试验结果为进一步进行微生物生产木聚糖酶的研究提供了理论依据。

The optimal medium and fermentation conditions of xylanase by MB22 were studied using single -factor-turn and orthogonal experiment. The results showed that the medium was composed of corncob meal120g/l,wheat bran280g/l,(NH4)2SO44g/l,CaCO31.5g/l,Tween80 2.0g/l,MgSO4·7H2O,1.5g/l,using corncob meal and wheat bran as carbon source.The optimum conditions were initial pH5.0,the temperature of the shaking table 30℃,rotating speed 160r/min, incubate time for 84h.Under these optimal conditions,the highest xylanase activity of 898.23U/ml of MB22 was obtained. Although this data needs to be increased compared with some higher yield strains,this result provided theoretical basis for further studies in producing xylanase by micro-organism.