老年人群网织红细胞参数的测定及初步临床应用

The measurement of reticulocytes and clinical applications in the elderly

目的 :确定健康老年人网织红细胞 (简称网红 )计数与分群的参考值范围 ,探讨其在老年贫血的诊断及白血病化疗中的临床应用价值。方法 :用BAYERADVIA 12 0自动血液分析仪检测了 338例健康老年人和 14 1例老年血液病患者的网红计数及分群 ,对数据进行了统计学处理。结果 :网红百分率 (RET)与绝对数 (RET # )和低、中、高荧光强度网红百分率 (LFR、MFR、HFR)在两性之间无显著性差异 (P >0 .0 5 ) ,结果 :0 .0 14 0± 0 .0 0 4 5、(6 2 .2 2±19.2 8)× 10 9/L、0 .85 71± 0 .0 4 4 1、0 .1190± 0 .0 336和 0 .0 2 39± 0 .0 139。老年贫血患者的RET #及HFR +MFR与正常参考值有显著性差异 (P <0 .0 5 )。白血病化疗后骨髓受抑 ,其外周血中的RET #及HFR +MFR极度减低 ;骨髓恢复期 ,HFR +MFR首先出现或升高。结论 :健康老年人网红计数及分群的参考值范围与性别无关 ;RET #及HFR

Objective:to establish a reference value of the reticulocytes counts and its subpopulation in the elderly, and study the value of its clinical applications in anemias diagnosis and leukemias chemotherapy.Methods:The reticulocytes were measured by automated hematology analyzer (ADVIA 120) with oxazine 750 as fluorochrome in 338 the healthy elderly and 141 aged hematological disorders. Results:The Student′s t-test showed that there were no significant differences(P>0.05)between male and female in the healthy elderly for reticulocyte percentage (RET) , reticulocyte absolute number(RET#), High fluorescence reticulocyt(HFR),middle fluorescence reticulocytes(MFR) and low fluorescence reticulocytes (LFR). They were 0.0140± 0.0045,(62.22±19.28)×10 9/L、0.8571±0.0441、0.1190±0.0336 and 0.0239±0.0139。There were significant differences(P<0.05)between anemias and reference value in the elderly for RET# and HFR+MFR. RET# and HFR+MFR was extremely decreased in leukemias after chemotherapy. HFR+MFR rise faster than RET# and the absolute neutrophil count (ANC)in bone marrow recovery. Conclusions:Reference value of the reticulocyte counts and its subpopulation were not correlated with sex in the healthy elderly. RET# and HFR+MFR have important significance in classification and difference diagnosis of anemias, estimation of bone marrow recovery after chemotherapy in the elderly. [