海水浸泡对兔颅脑挫裂伤Bcl-2和Bax蛋白表达的影响

Effect of seawater immersion on Bcl-2 and Bax protein expression of experimental brain contusion

目的 :建立海水浸泡颅脑挫裂伤模型 ,观察海水浸泡对实验性脑挫裂伤后创伤性脑水肿的影响及凋亡相关基因Bcl 2和Bax在蛋白表达水平变化。方法 :采用立体定向自由落体伤模型进行海水浸泡 30分钟作为实验组 ,对照组同样的方法致伤后不进行海水浸泡。观察创伤组织的病理改变 ,并通过免疫组化染色和计算机图像分析技术用半定量化的方法检测不同干预不同时程Bcl 2和Bax蛋白阳性反应强弱差异。结果 :实验组和对照组均发生了创伤性脑水肿 ,但水肿高峰期出现时间不一致 ,严重程度也不一致。实验组Bcl 2蛋白阳性表达强度低于对照组 ,Bax蛋白则高于对照组 ,Bcl 2 /Bax比值有降低趋势。结论 :海水浸泡影响了创伤性脑水肿进程 。

Objective:To build a seawater immerging brain contusion model,observe the effect of seawater on traumatic brain edema and alteration of protein expression of apoptosis-ralated genes Bcl-2 and Bax. Methods:Brain contusion area of stereostatic impact injuried adult rabbits were subjected to fresh seawater immerging for 30 minutes.We observed pathological changes of traunatic brain edema,and examined protein expression of apoptosis-related genes Bax and Bcl-2 by immunohistochemical staining. Results of immunohistochemical staining were analysed by computer image-analysis system.Results:Traumatic brain edema occured in both groups. Immunoreactivity of Bcl-2 protein in experimental group was lower than that in control group,but Bax protein immunoreactivity was higher,the ratio of Bal-2/Bax had a decreasing tendency.Conculsions:Seawater immerging has the aggravated effect on traumatic brain edema,and possibly promotes cells apoptosis around brain contusion area.