深低温停循环血液稀释时大鼠海马MDA、LDH和ATP酶的变化

Changes of hemodilution on MDA,LDH and ATPase of rat hippocampus after deep hypothermia circulation arrest

目的观察不同程度血液稀释对深低温停循环 (DHCA)大鼠海马丙二醛 (MDA)、乳酸脱氢酶 (LDH)和ATP酶的影响。方法建立大鼠DHCA (18℃ ,90min)模型 ,随机分成四组 :Hct 10 %组、Hct 2 0 %组、Hct 30 %组和假手术对照组。模型成功后取海马 ,应用比色法检测MDA、LDH和ATP酶的变化。结果Hct30 %组与Hct2 0 %组和Hct 10 %组相比 ,海马MDA含量最低 ,但仍然高于假手术组 ;LDH和ATP酶的活性最高 ,但仍低于假手术组。结论在DHCA期间保持较高的Hct值 (30 % )

Objective To observe the effects of various degrees of hemodilution on the malondialdehyde (MDA) lactate dehydrogenase(LDH) and adenosine triphosphate enzyme(ATPase) of hippocampus after deep hypothermia circulation arrest(DHCA) of rat. Methods The rat DHCA models were established and divided into four groups: Hct 10% group, Hct 20% group, Hct 30% group and sham operation group. After success of the model,hippocampuses were dissected to measure the changes of MDA, LDH and ATP enzyme with the colorimetric method. Results Comparing with the groups of Hct 10% and Hct 20%, the concentration of MDA in hippocampus of group Hct 30% was the lowest one,but higher than the sham operation group; the enzymatic activities of LDH and ATP of the group Hct 30% were the highest but still lower than those of the sham operation group. Conclusion Keeping higher Hct during DHCA does have the neuroprotective effect.