p53蛋白在口腔鳞癌中的表达

Study on the expression of p53 protein in oral squamous cell carcinoma

目的 通过对突变型p53蛋白在口腔鳞癌组织中表达的观察,分析p53与口腔鳞癌临床病理特征之间的关系,探讨p53在口腔鳞癌发生中的作用机制。方法 利用免疫组化(P-V法)检测116例患者口腔鳞癌组织及10例正常人口腔黏膜组织中突变型p53蛋白的表达情况。结果 p53蛋白在正常人口腔黏膜和口腔鳞癌组织中的表达率分别为20％、63.8％,二者比较差异有统计学意义(X2=5.660 4,P<0.05);口腔鳞癌在不同病理分级时,p53蛋白表达差异有统计学意义(X2=7.536 2,P<0.05);p53蛋白表达与年龄、性别、发生部位及有无淋巴结转移无相关性。结论 突变型p53蛋白表达与口腔鳞癌的发生有关。不同病理分级,p53蛋白表达也不同。

Objective To study the expression level of mutant p53 in oral squamous cell carcinoma as well as the relationship between mutant p53 and some clinical factors. Methods Immunohistochemistry P-V experiment was used for detection of mutant p53 protein. Results The positive expression rates of mutant p53 protein in normal oral mucosa and oral squamous cell carcinoma (OSCC) were 20% and 63.8%, respectively. Mutant p53 protein was demonstrated significantly (X2 = 5.660 4,P < 0.05) more often in OSCC than in normal oral mucosa and the situation was also found between histologic differentiation (X2 = 7.536 2,P < 0.05). While no significant correlations were found among patients'age, sex,tumor loci and metastasis status. Conclusions Mutant p53 protein plays a significant role in carcinoeenesis of OSCC.