环孢菌素A对体外NIT-1胰岛β细胞增殖及其增殖相关基因表达的影响

Effects of cyclosporine A on NIT-1 β cell proliferation and pol α1 gene expression in vitro

目的观察环孢霉素A(CsA)对体外培养的NIT-1胰岛β细胞增殖和poα1mRNA表达的影响.方法用MTT法检测不同浓度的CsA(0.05～10μmol/L)作用48和72h后NIT-1胰岛β细胞增殖的情况.利用半定量RT-PCR法检测10μmol/LCsA处理NIT-1胰岛β细胞48h后polα1在mRNA水平上的表达.结果CsA可抑制NIT-1胰岛β细胞的增殖,且与时间、剂量正相关.另外,CsA还下调了polα1mRNA的表达.结论CsA可抑制NIT-1胰岛β细胞增殖,其机制可能与下调polα1mRNA表达有关.

Objective To investigate the effects of cyclosporine A on the proliferation and pol α1 mRNA expression of cultured NIT-1 β cells. Methods After exposure to cyclosporine A at various concentrations (0.05 to 10 μmol/L) for 48 h and 72 h, NIT-1 cell proliferation was analyzed by MTT assay and the gene expression determined by reverse transcriptional PCR (RT-PCR). Results Forty-eight-hour and 72-hour cyclosporine A exposure inhibited the cell proliferation in a concentration- dependent manner, and at the concentration of 10 μmol/L, cyclosporine A also decreased pol α1 mRNA expression after a 48-hour exposure. Conclusion Cyclosporine A can effectively inhibit the proliferation of NIT-1 cells possibly through down-regulating the expression of pol α1 mRNA.