免疫低下小鼠轮状病毒肠道外感染的实验研究

Extraintestinal dissemination of rotavirus in immunodeficient mice

目的探讨免疫低下因素在诱发轮状病毒(RV)肠道外扩散中的作用,为临床防治提供参考后。方法给健康昆明小鼠注射环磷酰胺制作免疫低下小鼠模型,经口服和腹腔注射两种途径给免疫低下小鼠接种RV,处死小鼠,光镜下观察其病理变化。各脏器原位杂交,原位PCR检测RV。结果光镜下:口服RV组小肠绒毛、胃固有层、心肌细胞有改变;腹腔注射组除上述改变外,肝、肾也有改变。原位杂交:口服RV组小肠绒毛,腹腔注射组小肠绒毛、肾呈阳性。原位PCR:口服RV组小鼠的小肠绒毛,肠腺细胞,肾近曲小管和集合管上皮细胞呈阳性;腹腔注射组小鼠的肠、肾、肝、心、胰呈阳性;其余组动物各个组织器官均为阴性。结论免疫低下可能是诱发和加重RV 肠道外扩散和感染的重要原因之一。

Objective To investigate the extraintestinal dissemination of rotavirus (RV) in immunodeficient mice. Methods Immunodeficiency mouse model was established by injection of cyclophosphamide into the abdominal cavities of normal mice, then to which RV was administered either orally or intra-abdominally. The pathological changes in the organs were observed by light microscopy and RV was detected by in situ hybridization and PCR. Results Small intestinal villi, gastric lamina propria and cardiac myocytes exhibited pathological changes in the mice with oral RV administration. Besides these changes, the mice with intra-abdominal RV injection showed changes in the liver and kidneys. The intestinal villi of the mice with oral RV were RV positive by in situ hybridization. Positive results of RV in in situ PCR detection were found in the intestinal villi, intestinal gland cells, epithelial cells of the proximal convoluted tubules and collecting tubes in the kidney of the mice taken RV orally, and in the intestinal villi, kidneys, liver, heart and pancrease of mice with intra-abdominal RV injection. Conclusion Immunodeficiency may be the important factor for inducing and aggravating the infection and extraintestinal dissemination of RV.