放射诱导鼠脑神经元凋亡

Radiation-induced rat cerebral neuron apoptosis

目的探讨X射线诱导鼠脑神经元凋亡与照射剂量的关系及时间规律。方法将大鼠分为不同剂量照射组和对照组,以双标法计数凋亡的神经元数。结果X射线能诱导的鼠脑神经元的凋亡,凋亡率在照射前后有显著性差异(P<0.0001),在各剂量组间亦有显著性差异(P<0.005),同剂量组在照射后不同时间点间亦有显著性差异。结论低、中剂量X射线可诱导鼠脑神经元凋亡,凋亡率有剂量依赖性并具有时间规律性。

Objective To explore the correlation between X-ray-induced rat cerebral neuron apoptosis and radiation doses. Methods In a controlled experiment, the rats in the experimental group received X-ray radiation at different doses (2, 4, 6 and 8 Gy), and the apoptotic cerebral neurons were counted after in situ end-labeling and immunohistochemical staining. Results X-ray radiation induced apoptosis of rat cerebral neurons, and the apoptotic rate varied with the radiation doses. Significant difference in the apoptotic rate was noted before and after radiation (P<0.0001), and between different radiation dose groups (P<0.005). The apoptotic rate differed significantly at different time points after the radiation. Conclusion Low or medium doses of X-ray is able to induce rat cerebral neuron apoptosis depending on the radiation doses and following a definable time course.