摘要
AIM: Peroxisome proliferator-activated receptor γ (PPARγ) is known to regulate growbh arrest and terminal differentiation of adipocytes and is used clinically as a new class of antidiabetic drugs. Recently, several studies have reported that treatment of cancer cells with PPARγ ligands could induce cell differentiation and apoptosis, suggesting a potential application as chemopreventive agents against carcinogenesis. In the present study, 3 different kinds of PPARγ ligands were subjected to the experiments to confirm their suppressive effects on liver carcinogenesis.METHODS: Three PPARy ligands, pioglitazone (Pio) (200 ppm),rosiglitazone (Rosi) (200 ppm), and troglitazone (Tro) (1 000 ppm) were investigated on the induction of the placental form of rat glutathione S-transferase (rGST P) positive foci, a precancerous lesion of the liver,and liver cancer formation using a diethylnitrosamine-induced liver cancer model in Wistar rats, and dose dependency of a PPART ligand was also examined.RESULTS: PPART ligands reduced the formation of rGST P-positive foci by diethylnitrosamine and induction of liver cancers was also markedly suppressed by a continuous feeding of Pio at 200 ppm.CONCLUSION: PPART ligands are potential chemoprevenvea gents for liver carcinogenesis.
AIM:Peroxisome proliferator-activated receptor γ(PPARγ) is known to regulate growth arrest and terminal differentiation of adipocytes and is used clinically as a new class of antidiabetic drugs.Recently,several studies have reported that treatment of cancer cells with PPARy ligands could induce cell differentiation and apoptosis,suggesting a potential application as chemopreventive agents against carcinogenesis.In the present study,3 different kinds of PPARy ligands were subjected to the experiments to confirm their suppressive effects on liver carcinogenesis. METHODS:Three PPARy ligands,pioglitazone(Pio)(200 ppm), rosiglitazone(Rosi)(200 ppm),and troglitazone(Tro) (1000 ppm)were investigated on the induction of the placental form of rat glutathione S-transferase(rGST P) positive foci,a precancerous lesion of the liver,and liver cancer formation using a diethylnitrosamine-induced liver cancer model in Wistar rats,and dose dependency of a PPARy ligand was also examined. RESULTS:PPARy ligands reduced the formation of rGST P-positive loci By diethylnitrosamine and induction of liver cancers was also markedly suppressed by a continuous feeding of Pio at 200 ppm. CONCLUSION:PPARy ligands are potential chemopreventive agents for liver carcinogenesis.
基金
Supported by the National Natural Science Foundation of China,No.30371387
