视网膜Müller细胞条件培养基及抗VEGF对血管内皮细胞的作用

Effects of retinal Müller cell conditioned mediumand anti-VEGF on endothelial cells

目的 :观察高糖条件下制备的视网膜 Müller细胞条件培养基 (HGMCM)及抗 VEGF对血管内皮细胞的作用。方法 :采用免疫荧光、流式细胞术和 Boyden小室迁移实验观察 HGMCM,含 wortmannin及含 VEGF单克隆抗体 HGMCM对血管内皮细胞的作用。结果 :HGMCM可促进体外培养的血管内皮细胞的增殖和迁移(14 2 .0 0± 15 .32 /视野 ) ,wortm annin、VEGF单克隆抗体可明显抑制 HGMCM诱导的血管内皮细胞的增殖和迁移(89.0 0± 9.2 8/视野 ,93.0 0± 9.6 4 /视野 ) ,使血管内皮细胞被阻滞于 G1 / S转变期 ,含 wortm ennin和 VEGF单克隆抗体 HGMCM组与 HGMCM组比较 ,差异均有显著性 (P<0 .0 1)。结论 :HGMCM诱导血管内皮细胞的增殖和迁移一部分是通过 VEGF发挥作用的 ,拮抗 VEGF的活性可抑制血管内皮细胞的增殖和迁移 ,进而抑制新生血管形成。

Objective To investigate the effects of high glucose Müller cell conditioned medium(HGMCM) on endothelial cells. Methods Immunofluorescence, flow cytometry and Boyden chamber migration models were used to observe the effects of HGMCM,wortmannin and VEGF monoclone antibody on endothelial cells. Results HGMCM could accelerate the proliferation and migration of endothelial cells (142.00±15.32/per sight), wortmannin (89.00±9.28/per sight) and anti-VEGF antibody (93.00±9.64/per sight) could inhibit the proliferation and migration of endothelial cells induced by HGMCM, and endothelial cells were blocked in G 1/S phase. There were significant difference compared with HGMCM group (P<0.01). Conclusion One of reasons of the proliferation and migration of endothelial cells induced by HGMCM is the role of VEGF. The anti-VEGF can inhibit the proliferation and migration of endothelial cells to inhibite angiogenesis.