甲醛对小鼠肺组织形态及脂质过氧化物水平的影响

Effects of formaldehyde on lung histomophology and level of lipid peroxide in mice

目的 :探讨甲醛对小鼠肺组织形态及其脂质过氧化的影响。方法 :将 4 0只雄性健康小鼠随机分为 4组 :对照组和 3个甲醛染毒组 (2 0、 4 0和 80 m g· m- 3)进行静式染毒。每天 2 h连续染毒 5周后将小鼠全部处死 ,光镜下观察小鼠肺组织形态变化 ,并测定肺组织超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 :与对照组相比 ,光镜下可见吸入甲醛的小鼠肺毛细血管充血 ,肺泡间隔增宽 ,有炎细胞浸润 ,随着染毒剂量增加病理改变加重 ;2 0、 4 0和 80 m g· m- 3组小鼠肺组织 SOD活性明显降低 (P<0 .0 1) ,MDA含量明显升高 (P<0 .0 1) ,均呈剂量 -效应依赖的趋势 ,但组间比较差异无显著性 (P>0 .0 5 )。结论 :甲醛可损伤小鼠自由基清除系统。

Objective To study the effects of formaldehyde on lung histomorphology and the level of lipid peroxide in mice after inhaled different amount of formaldehyde. Methods Forty male mice were randomly divided into 4 groups: one control group and three formaldehyde dose groups(20,40, and 80 mg·m -3 ). The mice were poisoned 2 hours one day for 5 weeks continuously, then were killed. Lung tissues of mice inhaled formaldehyde were observed under light microscopy (LM). The activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) were detected biochemically in lung homogenate. Results It was manifestated as becoming bloodshots of pulmonary alveoli, expansion of pulmonary alveolus segment and inflammatory cells invasion under LM. The activities of SOD in 20,40, and 80 mg·m -3 dose groups were significantly lower than that in the control group, and the concentration of MDA was remarkably higher (P<0.01). The activity of SOD and the concentration of MDA had no remarkable differences between the different dose groups(P>0.05),but the former showed an increase activity in different dose groups, and the latter showed a reduced concentration in different dose groups. Conclusion Formaldehyde can injury free radical eliminated system to cause lung injury.