摘要
目的 :研究 Pin1(肽酰脯胺酰顺反式易构酶 )在髓系、淋系 10种恶性血液病细胞株中的表达情况以及其与不同细胞周期的关系。方法 :利用 Realtime- PCR检测血液病细胞株的 Pin1表达 ,同时用 Thymidine和 Nocoda-zole将细胞阻断在不同的细胞周期 ,用 Realtime- PCR及 Western Blot的方法 ,检测 Pin1的 m RNA和蛋白的表达水平。结果 :Pin1在恶性血液病细胞株中 m RNA的表达明显高于正常人骨髓单个核细胞的表达 (F=10 .5 0 8,P<0 .0 1) ,其在髓系血液病细胞株和淋系恶性血液病细胞株中的表达均较正常人骨髓单个核细胞明显增高 (分别为 F= 13.5 84 ,P<0 .0 1;F=5 .339,P<0 .0 5 )。 Pin1在 G1期 m RNA的表达水平显著高于 S期 (F=4 0 .5 72 ,P<0 .0 5 ) ,S期与 M期比较没有明显差异 (F=8.30 9,P>0 .0 5 )。但在蛋白水平上来看 Pin1的变化不明显。结论 :Pin1的 m R-NA在恶性血液病细胞中呈高表达 ,其表达水平与细胞周期有关 ,其中以 G1期为最高 ,S期最低。
Objective: To study the expression of peptidyl prolyl cis/trans isomerase (PPIase or Pin1) in malignant hematopoietic cells and its relation with cell cycle. Methods: Realtime quantitative PCR with fluorescence probe hybridization was used to measure expression of Pin1 mRNA in malignant hematopoietic cell lines and normal mononuclear cells separated from bone marrow.HeLa cells were blocked with Thymidine and Nocodazole in different cell phases and then the expression of Pin1 mRNA and protein were detected by realtime PCR and immunoblotting. Results: The expression of Pin1 in malignant hematopoietic cell lines was significantly higher than that in normal controls(0 339±0 093 vs 0 038±0 005, P <0 01). Its expression in myeloid malignant hemato poietic cell lines was significantly higher than that in normal controls (0 388±0 115 vs 0 038±0 005, P <0 01) and so was the malignant lymphocytic cell lines (0 226±0 166 vs 0 038±0 005, P <0 01).The expression of Pin1 was closely correlated with cell cycle.It was the highest in G1 phase and the lowest in S phase (110 762±16 737 vs 4 080±0 634, P <0 01). Conclusion: Pin1 is overexpressed in malignant hematopoietic cell lines and its expression is different during cell cycle that is highest in G1 phase and lowest in S phase. [
出处
《浙江大学学报(医学版)》
CAS
CSCD
2004年第6期500-503,514,共5页
Journal of Zhejiang University(Medical Sciences)
基金
国家重点基础研究发展计划 (973计划 )资助项目(2 0 0 2 CB71370 0 )
国家自然科学基金资助项目 (39870 339)