局灶性脑梗死引起谷氨酸转运体在星形胶质细胞的表达

THE EXPRESSION OF GLUTAMATE TRANSPORTER IN PLASTIC ASTROCYTES IN FOCAL CEREBRAL INFARCT

目的 探讨急性局灶性脑梗死可塑性变化的星形胶质细胞在脑缺血损伤中的作用。 方法 免疫组织化学和免疫荧光双标记技术。 结果 胶质原纤维酸性蛋白 (GFAP)阳性星形胶质细胞在脑梗塞灶的周围发生肥大和增生性可塑改变 ,其突起的变化尤为显著。粗大的突起呈纤维状 ,并相互交织呈密集的网 ,其末端向脑梗塞灶的中央延伸。在缺血周边的半影区可见谷氨酸转运体 (EAAT1)阳性表达 ,呈斑点和纤维状 ;共聚焦扫描显微镜下可见EAAT1与GFAP双标记的星形胶质细胞。 结论 急性局灶性脑梗死后发生可塑性变化的星形胶质细胞通过增强EAAT1的功能参与脑梗死的修复过程。

Objective To explore the role of plastic astrocytes in focal cerebral infarct. Methods Immunohistochemical and double immunofluorescence techniques were used in the study. Results The astrcytes positive for glial fibrillary acidic protein(GFAP) occurred in the periinfarct area appeared hypertrophy and proliferation,specially on their processes and formed a network,and the processes oriented toward the infarct center from the periinfarction.The expression of excitatory amino acid transporter 1( EAAT1) increased in the penumbra area of cerebral infarct and manifested spot and fibre.The confocal laser scanning microscopic analysis showed the double staining for EAAT1 and GFAP.Conclusion The plastic astrocytes might participate in the pathological process of cerebral infarct recovery by enhancing the expression of EAAT1.;