水通道蛋白4在大鼠眼球中的表达

THE EXPRESSION OF AQP4 IN RAT EYES

目的 从转录和翻译水平观察水通道蛋白 4 (AQP4 ) ,在正常Wistar大鼠眼球中的表达 ,为研究眼球中液体转运机制提供形态学基础。 方法 采用免疫组织化学SABC法和原位杂交技术。 结果 所测大鼠眼球呈较强的AQP4免疫反应阳性 ,角膜上皮细胞、虹膜色素上皮细胞、睫状体非色素上皮细胞、晶状体上皮细胞、晶状体纤维细胞、视网膜的内颗粒层、外颗粒层和节细胞层的细胞均为阳性细胞 ,免疫反应阳性物质主要分布在细胞膜上 ,胞核呈阴性。原位杂交结果同免疫组织化学染色基本一致 ,在以上细胞胞浆中均可检测到较强的AQP4mRNA阳性杂交信号 ,胞核呈阴性。 结论 AQP4可能在眼球中角膜和晶状体透明性的维持 ,房水的分泌和调节 ,视觉的激发和传递等功能活动中起着重要作用。

Objective To define the expression of AQP4 in normal rat eyes at transcriptional and translational level, so as to provide morphological bases for the mechanisms of water transport in eyes. Methods Immunohistochemical SABC method and in situ hybridization were used to detect the expression and localization of AQP4 in normal rat eyes. Results The corneal epithelium, the iridal pigment epithelium cells, the nonpigment epithelium cells of ciliary body, the lens epithelium cells, the lens fiber cells, the outer nuclear layer cells, and the inner nuclear layer cells, the ganglion cells of the retina showed positive immunoreactivity of AQP4. The reactive substance was distributed in membrane but not in nuclei of all positive cells. The resuIts of in situ hybridization were identical with that of immuohistochemistry method. AQP4 mRNA could be detected in the cytoplasm of positive immunoreactive cells, however, nuclei were negative. Conclusion The results implied that AQP4 could coordinate with other AQP4 to play an important role in the secretion and regulation of aqueous humor, the maintenance of the diaphaneity of cornea and lens, the motivation and transmission of optesthesia.;