常规染色体畸变分析法对AT细胞高辐射敏感性的研究

Study on High Radiosensitivity of AT Cells by Conventional Chromosome Aberration Analysis Method

目的 研究毛细血管扩张性共济失调症(ataxia-telangiectasia,AT)患者皮肤的成纤维细胞系AT5BIVA(AT细胞)的高辐射敏感性。方法 以源于正常人皮肤的成纤维细胞系GM0639(GM细胞)为对照,用常规染色体畸变分析法,在AT细胞和GM细咆经60COγ射线0、1、2、3、4 Gy照射后,观察比较AT细胞和GM细胞之间染色体畸变率(CAF)的差异,并分别进行曲线拟合。结果 在0、1、2、3、4 Gy剂量下,AT细胞染色体畸变率明显高于GM细胞(P<0.05);AT和GM细胞染色体畸变率与剂量成正相关,均可拟合成剂量效应直线方程Y=a+bX,且AT细胞剂量效应直线回归方程斜率明显大于GM细胞(P<0.05)。结论 AT患者AT细胞的辐射敏感性显著高于GM细胞,具有高辐射敏感性。

Objective To study the high radiosensitivity of fibroblast cells (AT5BIVA cells) from the skin of the patients of ataxia-telangiectasia (AT). Methods Using conventional chromosome aberraton analysis method, chromosome aberration frequence( CAF) of AT cells and GM cells exposed to 60CO γ-rays was observed at doses of 0, 1, 2, 3 and 4 Gg, compared with that of GM 0639(GM cells) from the skin of the normal subject. The difference of CAF between AT cells and GM cells was analyzed, and dose-response curves were established. Results After exposed to 0, 1,2,3 and 4 Gy 60 CO γ-rays, theradiation-induced level of CAF was significantly higher in AT cells compared with GM cells (P<0.05) . In the two cell types, CAF has a positive correlation with dose, and their linear regression equation is Y = a + bX. The slope of the CAF linear regression equation of AT cells is larger than that of GM cells (P<0.05). Conclusion The radiosensitivity of AT cells is significantly higher than that of GM cells. AT cells is extremely sensitive to radiation.