云锦杜鹃次生代谢产物质量分数的测定

Determination of content of secondary metabolites in Rhododendron fortunei

对云锦杜鹃Rhododendronfortunei不同大小级和不同营养器官的鞣质、生物碱、皂甙、木质素、绿原酸和大黄素等6种次生代谢产物的质量分数进行测定与分析。结果显示:6种次生代谢产物在云锦杜鹃不同营养器官中的质量分数具有一定的差异,总质量分数以茎最高,中树、小树、幼树分别为447 5g·kg-1,319 3g·kg-1和247 5g·kg-1;小树和幼树中叶片次之,分别为256 4g·kg-1和207 7g·kg-1,根最低,分别为234 8g·kg-1和174 7g·kg-1;中树中根次之,为344 5g·kg-1,叶最低,为317 0g·kg-1。6种次生代谢产物中,木质素的质量分数以茎最高,根次之,叶片最低;绿原酸的质量分数以根最高,叶片次之,而茎最低;而总生物碱、总皂甙、总鞣质、大黄素的质量分数均以叶片最高,茎次之,而根最低,其中大黄素的茎与根中的质量分数较为接近。6种次生代谢产物总量在3种器官中均以中树最高,小树次之,幼树最低,差异具有显著性意义。不同器官的6种次生代谢产物质量分数在不同大小级之间具有一定的差异。

The contents of 6 secondary metabolites such as tannin,saponin,alkaloid,lignin,emodin and chlorogenic acid in different organs of Rhododendron fortunei different class size were determined.The results showed that there were certain differences among the content of 6 secondary metabolites in different organs of Rhododendron fortunei.The toal content of 6 secondary metabolites was highest in stem.It was 447.5 g·kg^(-1)in middle sized tree,319.3 g·kg^(-1) in the little tree and 247.5 g·kg^(-1)in young tree,respectively.The total content in the little tree and young tree,took the second place with 256.4 g·kg^(-1)and 207.7 g·kg^(-1)respectively,while it in root was the lowest with 234.8 g·kg^(-1)and 174.7 g·kg^(-1)respectively.The total content in the root of the middle-sized tree took the second place 344.5 g·kg^(-1) while that in leaves was the lowest(317.0 g·kg^(-1)).The rank of contents of lignin was as follows:stem>root>leaves.The rank of chlorogenic acid was root>leaves>stem.The rank of the contents of the other 4 secondary metabolites was leaves>stem>root while the content of emodin in stem was as much as that in root.In three organs,the total content of 6 secondary metabolites in the middle-sized tree was highest,that in little tree took the secondary place while that in young tree was the lowest.In different organs,there were also certain differences among the contents of 6 secondary metabolites in Rhododendron fortunei with different sizes.