加减味耳聋左慈丸对耳蜗琥珀酸脱氢酶的保护作用

The protective effects of modifier Erlongzuociwan on cochlear succinate dehydrogenase

目的:观察中药方剂"耳聋左慈丸"加减味对庆大霉素(GM)耳毒性的防治作用,并探讨其作用机制。方法:用脑干听觉诱发电位(BAEP)的方法检测GM耳中毒豚鼠听阈的变化;用组织化学的方法检测耳蜗毛细胞线粒体内琥珀酸脱氢酶(SDH)的变化。结果:该中药方剂能降低GM引起的听觉反应阈的上升幅度,减轻GM对耳蜗毛细胞SDH损害,降低了对毛细胞的损伤。结论:该中药方剂具有明显保护耳蜗毛细胞SDH,降低GM的耳毒性的作用。保护耳蜗毛细胞线粒体SDH,维持了毛细胞的能量代谢及其功能,从而减轻了毛细胞损伤,这可能是该方剂降低GM耳毒性的机制之一。

Objective:To investigate the protective effects of modified Erlongzuociwan on the o-totoxicity of gentamycin (GM) and its mechanism. Methods: Changes in hearing threshold in guinea pigs with ototoxicity induced by GM were studied by brainstem auditory evoked potential(BAEP) and changes in succinate dehydrogenase (SDH) were studied by histochemistry.Results:Erlongzuociwan,a traditional Chinese medicine prescription, could decrease the elevated hearing response threshold and reduce SDH damage induced by GM. Conclusion: Erlongzuociwan can reduce the ototoxicity of GM and shows protective effect on SDH in cohlea hair cells.