S-西酞普兰草酸盐含量测定

Quantitative analysis of S-citalopram oxalate by chiral liquid chromatography

目的:拆分西酞普兰草酸盐对映异构体,建立S-西酞普兰草酸盐的质量检测方法。方法:采用Chiro-biotic V手性柱(250mm×4.6mm,5μm),以甲醇-冰醋酸-三乙胺(100:0.1:0.1)为流动相;检测波长为240nm,桂温为20℃,流速为1.0min·mL-1。结果:S型和R型异构体获得完全分离,S-西酞普兰草酸盐在10-150μg·mL-1范围内具有良好线性,r=0.999 1(n=5)。螬论:该方法简便、准确,可测定S-西酞谱兰草酸盐的含量。

Objective: To separate the enantiomers of citalopram oxalate and determinate S-citalopram axalate by HPLC. Methods: The chromatographic conditions were as follows: a Chirobiotic V column( 250mm×4. 6mm, 5μm), methanol-acetic acid-triethylamine (100:0.1:0.1) as a mobile phase,detection wavelength at 240nm, the column temperature at 201 and flow rate at 1.0mL·min-1. Results: The enantiomers of citalopram oxalate was successfully separated.The linearity of S-citalopram was 10-150μg·mL-1 with a regression coefficient at 0. 999 1 ( n = 5). Conclusion: This quantitative analysis of S-citalopram oxalate is attained.