乳康舒胶囊中活性物质的测定

Qualitative and quantitative determination of active ingredients in Rukangshu capsules

目的:研究乳康舒胶囊中活性物质淫羊藿苷、芍药苷及原儿茶醛的测定。方法:采用薄层色谱法对该复方中的淫羊藿、白芍、丹参药材进行色谱鉴别,HPLC法测定乳康舒胶囊中淫羊藿苷和芍药苷的含量。结果:该制剂中,淫羊藿、白芍、丹参有良好的展开系统,不存在阴性干扰。淫羊藿苷在92.4-3 326.4ng范围内呈良好的线性关系,r=0.999 9,回收率为100.4％,RSD为1.84％;芍药苷在93.0-3 348.0ng范围内有良好的线性关系.r=0.999 7,回收率为100.3％,RSD为1.46％。结论:本质量标准可有效地控制乳康舒胶囊的质量。

Objective:To study qualitative and quantitative determination of some active ingredients, such as icariin, paeoniflorin and protocatechualdehyde, in Rukangshu capsules. Methods: karri-in,paeoniflorin and protocatechualdehyde were identified by TLC technique and the content of icariin and paeoniflorin were determined by HPLC method. Results: Icariin, paeoniflorin and protocatechualdehyde were clearly separated in the TLC chromatogram. The results of HPLC analysis for icariin were: a linear range of 92.4-3 326. 4ng, r = 0.999 9, an average recovery of 100.4 % with a RSD of 1.84 % and the corresponding data for paeoniflorin were: 93.0 - 3 348. Ong, r=0. 999 7, an average recovery of 100.3 % with a RSD of 1.46 % . Conclusion: The qualitative and quantitative methods established are capable to be used for quality control of Rukangshu capsules.