两株抗小鼠胰腺癌McAb特性的初步研究

Preliminary Studies on Characteristics of Two Monoclonal Antibodies of Anti-Mouse Pancreatic Cancer

本文报告用盐折,DEAE纤维素层折或亲和层折法纯化2B_6,2B_(10)两株抗小鼠胰腺癌McAbs杂交瘤培养上清及其小鼠腹水中的Mcab,并经SDS-PAGE鉴定,FITC标记后,用直接荧光免疫法分析其在35例人类各种癌组织中相应抗抗原标记定位结果。2B_(10)与4例胃癌,2B_6,2B_(10)与3例卵巢癌组织,1株卵巢癌细胞株呈强阳性反应。表明人类与小鼠之间存在共同肿瘤抗原。

Two monoclonal antibodies of anti-mouse pancreatic cancer, 2B_6and 2B^(10), were purified by the use of salt fractionation-exchange chromatography(DEAE-52) or affinity chromatography from culture supernatants and mice ascites of the hybridomas. After assayed by the use of SDS-PAGE and labeled with FITC, they were used to analyse related antigen site of 35 tissue samples of several human tumor by means of direct-fluorescence method. There was an intense positive reaction between 2B_(10)and 4 samples of gastric cancer. 2B_6or 2B_(10)and 3 tissur samples of ovary cancer and 1 clonal cell of ovary cancer also had intense positive reaction. These results showed that there exist common tumor antigen in both human and mice.