人早孕期蜕膜及绒毛膜组织PRL和HPL的免疫组化定位

Immunohistolochemical Localization of PRL and HPL in Human Decidua and Chorion of Early Pregnancy

本研究用免疫组化方法观察了蜕膜组织及绒毛膜组织中的催乳索(Prolactin, PRL)和胎盘催乳素(Human placenta lactogen HPL)的分布。并用正常已婚未孕妇女子宫内膜对HPL作对照。结果:已婚未孕妇女子宫内膜HPL为阴性。②脱膜组织中蜕膜细胞的胞质对PRL显阳性,绒毛膜组织对PRL显阴性。③绒毛膜组织中合体滋养层细胞的胞质对HPL显强阳性。④某些部位蜕膜细胞之间分散或成群的小细胞对HPL显阳性。⑤侵入蜕膜组织中的绒毛外滋养层细胞的胞质对HPL显强阳性。⑥值得注意的是HPL在蜕膜组织的血管壁各层细胞中均呈阳性反应。特别是毛细血管内皮细胞对HPL的阳性反应,目前尚未见报道,有待进一步研究。

By the means of immunohistology we have observed the distribution of prolactin (PRL) and human placenta lactogen (HPL) in decidua and ehorion. The results are as followe: (1) HPL was negatively stained in normal nonpregnant endometria; (2)PRL staining showed posi- tive in cytoplasm of decidual cells whereas negative in chorionic villi; (3) syncytiotrophoblast cells of chorionic villi were positive for villi; (4) some small cells scattered between decidual cells were positive for HPL; (5)extravillous trophoblastic infiltrated in decidua were positively stained with HPL; (6)it is interesting to note that HPL positive cells could be present among the cells of blood vessel wall, especially in endothelial cells of capillaries, about which there is no report so far. The significance of HPL positive cells in decidua deserves further study.